Блог около Bioneer Launches PCR Kit for Marine Pathogen Detection in Aquaculture

In the vast and mysterious world of the ocean, an unseen war rages on. A pathogen called Vibrio harveyi silently threatens marine life, destabilizing global aquaculture industries. This Gram-negative bacterium, while harmless to humans, acts as a deadly "invisible killer" for shrimp, fish, and other marine organisms. It causes symptoms such as eye lesions, muscle necrosis, skin ulcers, and fin deformities, leading to significant economic losses for farmers and posing ecological risks. However, due to the nonspecific nature of these symptoms, visual diagnosis is unreliable, creating an urgent need for highly sensitive detection methods.

Solving the Diagnostic Challenge: The Birth of AccuPower® Vibrio harveyi Real-Time PCR Kit

Addressing this challenge, innovators in life sciences have developed a breakthrough solution. Bioneer's AccuPower® Vibrio harveyi Real-Time PCR Kit leverages molecular diagnostics to detect the pathogen with precision, safeguarding aquaculture health.

Revolutionary Technology for Accurate Detection

The kit's core advantage lies in its exceptional sensitivity and specificity, powered by Bioneer's proprietary PyroHotStart® technology. This enzyme-mediated hot-start mechanism suppresses nonspecific amplification during PCR initiation, minimizing background noise and ensuring efficient target gene amplification. Even with minimal DNA templates, the kit reliably identifies V. harveyi with zero tolerance for false negatives.

Streamlined Workflow with "All-in-One" Design

Designed for efficiency, the kit features a pre-mixed, single-tube format containing all essential reagents—dNTPs, PCR buffer, magnesium ions, and hot-start DNA polymerase. Users need only add template DNA and DEPC-treated water to initiate the reaction. This simplified process reduces experimental errors, enabling even novice researchers to achieve consistent, reproducible results.

Rigorous Quality Control and Applications

Manufactured under ISO 9001 standards, the kit ensures batch-to-batch reliability. Its applications span multiple critical areas in aquaculture:

• Early Warning & Disease Prevention: Routine screening of water, broodstock, or larvae detects V. harveyi before outbreaks occur.
• Diagnosis: Rapid, accurate identification of the pathogen guides targeted treatment during suspected infections.
• Breeding Stock Inspection: Ensures pathogen-free genetic resources to prevent transmission.
• Environmental Monitoring: Assesses water quality to optimize farming conditions.

Validated Performance with Experimental Data

Bioneer's validation tests, conducted on the Exicycler™ 96 Real-Time PCR system, demonstrate the kit's accuracy through clear amplification curves and reliable Ct values.

Product Specifications

The kit includes:

• 2X Master Mix (2 x 625 μl): Pre-optimized PCR components.
• Oligo Mix (500 μl): Target-specific primers and probes.
• DEPC-D.W. (1.8 ml): Nuclease-free water.
• Positive Control (50 μl): High-copy reference for validation.

Catalog Number: K-2980
Size: 1.25 ml of 2X Master Mix (100 tests)